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RNDr. Ján Štěrba, Ph.D.


Projects

co-PI of project of Grant Agency of Academy of Sciences of the Czech Republic: Glycoprotein analysis of Ixodes ricinus salivary glands (2009-2011)

 PI of grants of the Grant Agency of University of South Bohemia: Glycosylation of tick-borne encephalitis virus envelope protein and its influence on virus virulence (2006), Glycosylation of membrane proteins of Lyme borreliosis spirochetes (2007)

 co-PI of two projects of Grant Agency of University of South Bohemia and of The Agency of the Council of Higher Education Institutions (2008);

 participation on several projects of Grant Agency of Academy of Sciecens of the Czech Republic, Grant Agency of the Czech Republic, Ministry of Education, Youth and Sports

Teaching

Biochemické praktikum pro Biologii a ŽP (UCH/051) - winter term, in Czech

 Basic laboratory course in Biochemistry for students of study programmes not oriented to Chemistry, Molecular Biology, and Medicinal Chemistry.

 

Praktikum z biochemie (UCH/049) - winter term, in Czech

Basic laboratory course in Biochemistry for students of study programmes oriented to Chemistry, Molecular Biology, and Medicinal Chemistry.

 

Biochemistry Laboratory 1 (UCH/758) - winter term, in English

Basic laboratory course in Biochemistry for students of the Biological Chemistry study programme.

 

Pokročilé praktikum z biochemie (UCH/050) - summer term, in Czech

Advanced laboratory course in Biochemistry for students of the Chemistry study programme.

Biochemistry Laboratory 2 (UCH/642) - summer term, in English

Advanced laboratory course in Biochemistry for students of the Biological Chemistry study programme.

Farmakologie a toxikologie (KME/748) - winter term, in Czech

Course offered especially for the students of the study programmes Biomedicinal Laboratory technique and Clinical Biology. Basics in pharmacology, mode of action of drugs on molecular level, basics in pharmacogenetics,clinical trials, basics in toxicology and xenobiochemistry, action of toxic substances on molecular level. Lab. course: pharmacogenetics in practice - cytochromes and aldehyde- and alcohodehydrogenase.

Úvod do toxikologie (UCH/112) - winter term, in Czech

Course offered especially for the students of the Chemistry study programme. Basics in toxicology, action of toxic substances on molecular leve, reactions in detoxification - Phase I and Phase reaction in xenobiochemistry. Lab. course: toxicogenetics in practice - cytochromes and aldehyde- and alcohodehydrogenase.

Xenobiochemistry and Toxicology (UCH/052) - summer term, in English

Course offered especially for the students of the Biological Chemistry study programme. Basics in toxicology, action of toxic substances on molecular leve, reactions in detoxification - Phase I and Phase reaction in xenobiochemistry. Lab. course: toxicogenetics in practice - cytochromes and aldehyde- and alcohodehydrogenase.

Supervised BSc. and MSc. theses

Published scientific papers

Růžek D., Kopecký J., Štěrba J., Golovchenko M., Rudenko N., Grubhoffer L.: Non-virulent strains of TBE virus circulating in the Czech Republic. Journal of Clinical Virology 36 (suppl. 2): S41, 2006 (P117).

Růžek D., Štěrba J., Kopecký J., Grubhoffer L.: The supposedly attenuated Hy-HK variant of highly virulent Hypr strain of tick-borne encephalitis virus is obviously a stain of Langat virus. Acta Virologica 50(4), 227-278, 2006.

Štěrba J., Vancová M., Rudenko N., Golovchenko M., Tremblay T.-L., Kelly J.F., MacKenzie R.C., Logan S.M., Grubhoffer L.: Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated. Journal of Bacteriology 190: 2619-2623, 2008.

Man P., Kovář V., Štěrba J., Strohalm M., Kavan D., Kopáček P., Grubhoffer L., Havlíček V.: Deciphering Dorin M glycosylation by mass spectrometry. European Journal of Mass Spectrometry 14: 345-354, 2008.

Štěrba J., Dupejová J., Fišer M., Golovchenko M., Rudenko N., Grubhoffer L.: Identification and characterisation of lectins in several tick species. FEBS Journal, 276 (Suppl. 1): 88, 2009.

Grubhoffer L., Hajdušek O., Vancová M., Štěrba J., Rudenko N.: Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans. FEBS Journal, 276 (Suppl. 1): 141, 2009.

Dupejova J., Sterba J., Vancova M., Grubhoffer L.: Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization. Parasites&Vectors, 4:4, 2011.

Sterba J., Dupejova J., Fiser M., Vancova M., Grubhoffer L.: Fibrinogen-related proteins in ixodid ticks. Parasites&Vectors, 4:127, 2011.

Vancova M., Sterba J., Dupejova J., Simonova Z., Nebesarova J., Novotny M.V., Grubhoffer L.: Uptake and incorporation of sialic acid by the tick Ixodes ricinus. Journal of Insect Physiology, 2012, 58, 1277-1287.

Grubhoffer L., Rudenko N., Vancova M., Golovchenko M., Sterba J.: Circulatory system and hemolymph. In: Biology of ticks. Vol. 1. Daniel E. Sonenshine (Editor), R. Michael Roe (Editor). Oxford University Press Inc; 2013, 2nd Revised edition. pp. 258-286.

Sterba J., Vancova M., Sterbova J., Bell-Sakyi L., Grubhoffer L.: The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick. Carbohydrate Research, 2014, 389, 93-99.

Presentations at conferences:

2014

13th Bratislava Symposium on Saccharides, Smolenice, Slovakia, lecture: „The origin of sialic acid in adult ticks and its localization“; poster: „Localization and identification of fucosylated glycoproteins in Ixodes ricinus ticks using bioorthogonal chemistry“.

4th Visegrad Symposium on Structural Systems Biology, Nové Hrady, Czech Republic, lecture: „Glycosylation – from proteins to an organism“.

2013

17th European Carbohydrate Symposium (EuroCarb 17), Tel-Aviv, Israel, two posters: „Majority of sialylated glycoproteins in adult ticks originates in the host, not the tick“,„Fucosylation in Ixodes ricinus tick: localization and purification of fucosylated glycoproteins using bioorthogonal chemistry“.

2012

XXIII. Biochemický sjezd, Brno, Czech Republic, lecture: „Advances in tick glycobiology“; poster: „All that glitters is not gold … or glycan. (Detection of glycans in Ixodes ricinus tick cell cultures and tissues using bioorthogonal chemistry)“.

2011

XI. Sigma-Aldrich konference mladých biochemiků a molekulárních biologů, Žďár n. Sázavou, ČR: lecture “N-glycans in saliva and salivary glands of the tick Ixodes ricinus”;

Ticks and Tick-borne Pathogens Conference 7, Zaragoza, Spain: “Ornithodoros moubata cell culture producing the tick lectin, Dorin M”, Sterba J., Dupejova J., Vancova M., Palus M., Bell-Sakyi L., Grubhoffer L.; “Characterization of the hemelipoglycoprotein from the tick, Dermacentor marginatus”, Dupejova J., Sterba J., Vancova M., Grubhoffer L.; “Mass spectrometric identification and electron microscopy localization of sialic acids in Ixodes ricinus salivary glands”, Vancova M., Sterba J., Dupejova J., Nebesarova J., Novotny M.V., Grubhoffer L.; “Glycobiology of ticks” Grubhoffer L., Dupejova J., Vancova M., Fiser M., Novotny M.V., Sterba J.

XV. Setkání biochemiků a molekulárních biologů, Brno, ČR: “Core-fucosylation in Ixodes ricinus ticks: Life-stage- and structure-specific detection”, Dupejova J., Sterba J., Vancova M., Novotny M.V., Grubhoffer L.

2010

25th international symposium on Microscale bioseparations MSB 2010, 21.-25.3.2010: Sterba J. Vancova M., Alley WR, Mann BF, Bucinska L, Dupejova J, Grubhoffer L, Novotny MV P195: Analysis of minute volumes of tick saliva samples using MALDI TOF/TOF mass spectrometry, p.158

XIV. Setkání biochemiků a molekulárních biologů, 20.-21.4.2010: Štěrba J., Vancová M., Alley WR Jr., Mann BF, Bučinská L, Dupejová J, Grubhoffer L, Novotný MV N-glycans from the saliva and salivary glands of the tick Ixodes ricinus, p.39-40

2009

Curent chemistry and biochemistry of carbohydrates - Cukrblik 2009, 16.4.2009: Štěrba J., Man P., Kovář V., Dupejová J., Fišer M., Strohalm M., Kavan D., Kopáček P., Vancová M., Havlíček V., Grubhoffer L. Fibrinogen-related proteins/lectins in ticks, p. 17; Vancová M., Bučinská L., Štěrba J., Nebesářová J., Grubhoffer L. The presence of sialic acid in the salivary glands of the tick Ixodes ricinus, p.35

2008

XXI. Biochemický sjezd: Grubhoffer L, Hajdušek O, Vancová M, Štěrba J, Rudenko N O97 Glykobiochemie klíšťat – přenašečů závažných onemocnění: sacharidy vázající proteiny a glykany, p.187; Vancová M, Bučinská L, Štěrba J, Grubhoffer L P167 The presence of N-acetylneuraminic acid modified protein in salivary glands of ticks Ixodes ricinus, p.192; Dupejová J, Śtěrba J, Vancová M, Kovář V, Grubhoffer L P115 Fibrinogen-related proteins (elctins) in tick Dermacentor marginatus hemolymph, p.136

2007

Structure and stability of biomacromolecules SSB 2007, 5.-8.9.2007: Štěrba J, Vancová M, Rudenko N, Golovchenko M, Kelly JF, MacKenzie RM, Logan SM, Grubhoffer L PO35 Structural characterization of outer surface (Osp) and flagellin (Fla) proteins from Borrelia burgdorferi, p.121

8th Multinational Congress on Microscopy, 17.-21.6.2007: Vancová M, Nebesářová J, Vaněček J, Štěrba J, Fránová J LP-58 Observation of Flagella and Viruses in Low-Voltage Transmission Electron Microscope, p.545

2006

XX. biochemický zjazd, 12.-16.9.2006: Štěrba J, Vancová M, Rudenko N, Golovchenko M, Kelly J, Logan S, MacKenzie R, Grubhoffer L PO9.13 Glycosylation in Lyme borreliosis spirochetes, p.396