Katedra molekulární biologie a genetiky
Department of molecular biology and genetics

A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance

Petr Nguyen and Martina Dalíková from the Faculty of Science, University of South Bohemia in České Budějovice and the Biology Center AS CR and their colleagues from a large international scientific team mapped the arrangement of the codling moth Cydia pomonella genome at chromosome level, which includes sex chromosome Z and part of sex chromosome W. Their research was published in Nature Communications, congratulations!

Their aim was to contribute to answering key questions about the moth, including the reasons for its success in spreading and invading orchards around the world, the mechanisms of its increasing resistance to the insecticides used, as well as the genetic nature of the rapid adaptation of the moth to very different environments.

Figure: Fanghao Wan et al. 2019

The codling moth Cydia pomonella, a major invasive pest of pome fruit, has spread around the globe in the last half century. We generated a chromosome-level scaffold assembly including the Z chromosome and a portion of the W chromosome. This assembly reveals the duplication of an olfactory receptor gene (OR3), which we demonstrate enhances the ability of C. pomonella to exploit kairomones and pheromones in locating both host plants and mates. Genome-wide association studies contrasting insecticide-resistant and susceptible strains identify hundreds of single nucleotide polymorphisms (SNPs) potentially associated with insecticide resistance, including three SNPs found in the promoter of CYP6B2. RNAi knockdown of CYP6B2 increases C. pomonella sensitivity to two insecticides, deltamethrin and azinphos methyl. The high-quality genome assembly of C. pomonella informs the genetic basis of its invasiveness, suggesting the codling moth has distinctive capabilities and adaptive potential that may explain its worldwide expansion.
source:  Fanghao Wan et al. 2019

Wan, F., Yin, C., Tang, R., Chen, M., Wu, Q., Huang, C., Qian, W., Rota-Stabelli, O., Yang, N., Wang, S., Wang, G. Zhang, G., Guo, J., Gu, L., Chen, L., Xing, L., Xi, Y., Liu, F., Lin, K., Guo, M., Liu, W., He, K., Tian, R., Jacquin-Joly, E., Franck, P., Siegwart, M., Ometto, L., Anfora, G., Blaxter, M., Meslin, C., Nguyen, P., Dalíková, M., Marec, F., Olivares, J. Maugin, S., Shen, J., Liu, J., Guo, J., Luo, J., Liu, B., Fan, W., Feng, L., Zhao, X., Peng, X., Wang, K., Liu, L., Zhan, H., Liu, W., Shi, G., Jiang, C., Jin, J., Xian, X., Lu, S., Ye, M., Li, M., Yang, M., Xiong, R., Walters, J. R., Li, F. 2019. A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ekology and insecticide resistence. Nature Communications 10: 4237.