RNDr. Alena Krejčí, Ph.D.


WORK ADDRESS: University of South Bohemia, Faculty of Science / Biology Centre, Institute of Entomology, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic

Telephone: +420 (0)38 777 2270

E-mail: akrejci@prf.jcu.cz





EDUCATION
:   2004 -  Ph.D. in molecular genetics at Faculty of Science, Charles University, Prague

WORK EXPERIENCE & RESEARCH HISTORY:

May 2010 – present: University of South Bohemia, Faulty of Science / Biology Centre, Institute of Entomology, Ceske Budejovice, Czech Republic. Group leader of the Laboratory of Developmental Biology

2004 – 2010: Department of Physiology, Development and Neuroscience, University of Cambridge, United Kingdom. Senior Research Associate at the laboratory of Prof. Sarah Bray

2001- 2004: Royal Society fellowship at the University of Leeds, School of  Biochemistry and Molecular Biology, United Kingdom. Laboratory of  Prof. Noel Buckley. 

1998 - 2004: Department of Neurochemistry, Institute of Physiology, Academy of Sciences, Prague. Laboratory of Prof. Stanislav Tucek and Dr. Vladimir Dolezal. Graduate and Ph. D. studies

GRANTS:

  • Grant from the Czech Grant Agency, 2017-2019: Metabolic stress as regulator of Notch signalling.
  • Grant from the Czech Grant Agency, 2014-2016: The role of sirtuins in Notch signalling.
  • Grant from the Czech Grant Agency, 2011-2013: Signallization through the Notch receptor in the context of cellular metabolism.
  • EMBO installation grant, 2011-2016: The interplay between Notch signalling and cellular metabolism. Funding of 250k euro total.

AWARDS and FELLOWSHIPS:

  • Early Research Career Award from the British Biochemical Society for the year 2011.
  • Award for the best popular science article in the magazine 'Ziva' (in Czech, artical about the Warburg effect)
  • Winner of the L'Oreal Women in Science award in the Czech Republic, 2011.
  • EMBO long term fellowship, March 2005 - March 2007, laboratory of prof. Sarah Bray, University of Cambridge, UK
  • Royal Society fellowship, 10 months in total between 2001 – 2004. Laboratory of Prof. Noel Buckley, School of Biochemistry and Molecular Biology, University of Leeds, UK

 

PUBLICATIONS:

  • Horvath M, Mihajlovic Z, Slaninova V, Perez-Gomez R, Moshkin Y, Krejci A: The silent information regulator 1 (Sirt1) is a positive regulator of the Notch pathway in Drosophila. Biochemical journal 473(22):4129-4143, 2016.
  • Slaninova V, Krafcikova M, Perez-Gomez R, Steffal P, Trantirek L, Bray SJ and Krejci A: Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. Open Biology, 2016 Feb;6(2)., 2016. doi: 10.1098/rsob.150155.
  • Housden BE, Fu AQ, Krejci A, Bernard F, Fischer B, Tavare S, Russell S, Bray SJ: Transcriptional Dynamics Elicited by a Short Pulse of Notch Activation Involves Feed-Forward Regulation by E(spl)/Hes Genes. Plos Genetics, 9(1):e1003162, 2013.
  • Djiane A, Krejci A, Bernard F, Fexova S, Millen K, Bray SJ.: Dissecting the mechanisms of Notch induced hyperplasia.  EMBO Journal 32, 60 - 71, 2013. doi:
  • Endo K, Karim MR, Taniguchi H, Krejci A, Kinameri E, Siebert M, Ito K., BraySJ, Moore AW: Chromatin modification of Notch targets in olfactory receptor neuron diversification. Nature Neuroscience, 15(2):224-33, 2012. doi: 10.1038/nn.2998.
  • Terriente-Felix A, Li J, Collins S, Mulligan A, Reekie I, Bernard F, Krejci A,  Bray SJ: Notch co-operates with Lozenge/Runx to lock hemocytes into a differentiation programme. Development, 140(4):926-37, 2013. doi: 10.1242/dev.086785
  • Bernard F, Krejci A, Housden B, Adryan B, Bray SJ: Specificity of Notch pathway activation: twist controls the transcriptional output in adult muscle progenitors. Development, 137(16):2633-42, 2010. doi: 10.1242/dev.053181 
  • Krejci A*, Bernard F*, Housden BE*, Collins S, Bray SJ.: Direct response to Notch activation: signaling crosstalk and incoherent logic. Science Signal.,2(55), 2009. doi:10.1126/scisignal.2000140
  • Narasimha M, Uv A, Krejci A, Brown NH, Bray SJ.: Grainy head promotes expression of septate junction proteins and influences epithelial morphogenesis. J Cell Sci. 2008. doi: 10.1242/jcs.019422
  • Goodfellow H*, Krejcí A*, Moshkin Y*, Verrijzer CP, Karch F, Bray SJ.: Gene-specific targeting of the histone chaperone asf1 to mediate silencing. Dev Cell.13(4):593-600, 2007.PMID: 17925233
  • Krejci A and Bray S: Notch activation stimulates transient and selective binding of Su(H)/CSL to target enhancers.  Genes Dev. 21(11):1322-7, 2007. PMID: 17545467
  • Bruce AW, Krejci A, Ooi L, Deuchars J, Wood IC, Dolezal V, Buckley NJ. :The transcriptional repressor REST is a critical regulator of the neurosecretory phenotype. J Neurochem. 98(6):1828-40, 2006. PMID: 16945103
  • Nagel AC, Krejci A, Tenin G, Bravo-Patino A, Bray S, Maier D, Preiss A. : Hairless-mediated repression of notch target genes requires the combined activity of Groucho and CtBP corepressors. Mol Cell  Biol. 25(23):10433-41, 2005. PMID: 16287856
  • Jakubik J, Krejci A, Dolezal V: Asparagine, valine, and threonine in the third extracellular loop of muscarinic receptor have essential roles in the positive cooperativity of strychnine-like allosteric modulators. J Pharmacol Exp Ther. 313(2):688-96, 2005.
  • Krejci A, Bruce AW, Tucek S, Dolezal V, Buckley NJ: Multiple promoters drive tissue specific expression of the human M2 muscarinic acetylcholine receptor gene. J Neurochem. 91(1):88-98, 2004.
  • Tucek S, Krejci A, Lysikova M, Jakubik J, Dolezal V: Roles of external loops of muscarinic receptors in interactions between N-methylscopolamine and allosteric modulators. In Cholinergic mechanisms: Function and disfunction. Ed. I. Silman, 71-76, 2004.
  • Krejci A, Michal P, Jakubik J, Ricny J, Dolezal V: Regulation of signal transduction at M2 muscarinic receptor. Physiol. Res., 53, suppl.1:S131-40, 2004.
  • Krejci A, Tucek S: Quantitation of mRNAs for M1 to M5 subtypes of muscarinic receptors in rat heart and brain cortex. Mol Pharmacol.  61(6):1267-72, 2002.
  • Krejci A, Tucek S: Changes of cooperativity between N-methylscopolamine and allosteric modulator alcuronium and gallamine induced by mutations of external loops of muscarinic M3 receptors. Mol. Pharmacol. 60 : 761-767, 2001.

ORCID number: 0000-0001-7589-4035 

H-index:  15