RNDr. Alena Krejčí, Ph.D.
WORK ADDRESS: University of South Bohemia, Faculty of Science / Biology Centre, Institute of Entomology, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic
Telephone: +420 (0)38 777 2270
EDUCATION: 2004 - Ph.D. in molecular genetics at Faculty of Science, Charles University, Prague
WORK EXPERIENCE & RESEARCH HISTORY:
May 2010 – present: University of South Bohemia, Faulty of Science / Biology Centre, Institute of Entomology, Ceske Budejovice, Czech Republic. Group leader of the Laboratory of Developmental Biology
2004 – 2010: Department of Physiology, Development and Neuroscience, University of Cambridge, United Kingdom. Senior Research Associate at the laboratory of Prof. Sarah Bray
2001- 2004: Royal Society fellowship at the University of Leeds, School of Biochemistry and Molecular Biology, United Kingdom. Laboratory of Prof. Noel Buckley.
1998 - 2004: Department of Neurochemistry, Institute of Physiology, Academy of Sciences, Prague. Laboratory of Prof. Stanislav Tucek and Dr. Vladimir Dolezal. Graduate and Ph. D. studies
- Grant from the Czech Grant Agency, 2017-2019: Metabolic stress as regulator of Notch signalling.
- Grant from the Czech Grant Agency, 2014-2016: The role of sirtuins in Notch signalling.
- Grant from the Czech Grant Agency, 2011-2013: Signallization through the Notch receptor in the context of cellular metabolism.
- EMBO installation grant, 2011-2016: The interplay between Notch signalling and cellular metabolism. Funding of 250k euro total.
AWARDS and FELLOWSHIPS:
- Early Research Career Award from the British Biochemical Society for the year 2011.
- Award for the best popular science article in the magazine 'Ziva' (in Czech, artical about the Warburg effect)
- Winner of the L'Oreal Women in Science award in the Czech Republic, 2011.
- EMBO long term fellowship, March 2005 - March 2007, laboratory of prof. Sarah Bray, University of Cambridge, UK
- Royal Society fellowship, 10 months in total between 2001 – 2004. Laboratory of Prof. Noel Buckley, School of Biochemistry and Molecular Biology, University of Leeds, UK
- Krejci A, Tennessen JM.: Metabolism in time and space - exploring the frontier of developmental biology. Development 144(18):3193-3198, 2017.
- Horvath M, Mihajlovic Z, Slaninova V, Perez-Gomez R, Moshkin Y, Krejci A: The silent information regulator 1 (Sirt1) is a positive regulator of the Notch pathway in Drosophila. Biochemical journal 473(22):4129-4143, 2016.
- Slaninova V, Krafcikova M, Perez-Gomez R, Steffal P, Trantirek L, Bray SJ and Krejci A: Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. Open Biology, 2016 Feb;6(2)., 2016. doi: 10.1098/rsob.150155.
- Housden BE, Fu AQ, Krejci A, Bernard F, Fischer B, Tavare S, Russell S, Bray SJ: Transcriptional Dynamics Elicited by a Short Pulse of Notch Activation Involves Feed-Forward Regulation by E(spl)/Hes Genes. Plos Genetics, 9(1):e1003162, 2013.
- Djiane A, Krejci A, Bernard F, Fexova S, Millen K, Bray SJ.: Dissecting the mechanisms of Notch induced hyperplasia. EMBO Journal 32, 60 - 71, 2013. doi:
- Endo K, Karim MR, Taniguchi H, Krejci A, Kinameri E, Siebert M, Ito K., BraySJ, Moore AW: Chromatin modification of Notch targets in olfactory receptor neuron diversification. Nature Neuroscience, 15(2):224-33, 2012. doi: 10.1038/nn.2998.
- Terriente-Felix A, Li J, Collins S, Mulligan A, Reekie I, Bernard F, Krejci A, Bray SJ: Notch co-operates with Lozenge/Runx to lock hemocytes into a differentiation programme. Development, 140(4):926-37, 2013. doi: 10.1242/dev.086785
- Bernard F, Krejci A, Housden B, Adryan B, Bray SJ: Specificity of Notch pathway activation: twist controls the transcriptional output in adult muscle progenitors. Development, 137(16):2633-42, 2010. doi: 10.1242/dev.053181
- Krejci A*, Bernard F*, Housden BE*, Collins S, Bray SJ.: Direct response to Notch activation: signaling crosstalk and incoherent logic. Science Signal.,2(55), 2009. doi:10.1126/scisignal.2000140
- Narasimha M, Uv A, Krejci A, Brown NH, Bray SJ.: Grainy head promotes expression of septate junction proteins and influences epithelial morphogenesis. J Cell Sci. 2008. doi: 10.1242/jcs.019422
- Goodfellow H*, Krejcí A*, Moshkin Y*, Verrijzer CP, Karch F, Bray SJ.: Gene-specific targeting of the histone chaperone asf1 to mediate silencing. Dev Cell.13(4):593-600, 2007.PMID: 17925233
- Krejci A and Bray S: Notch activation stimulates transient and selective binding of Su(H)/CSL to target enhancers. Genes Dev. 21(11):1322-7, 2007. PMID: 17545467
- Bruce AW, Krejci A, Ooi L, Deuchars J, Wood IC, Dolezal V, Buckley NJ. :The transcriptional repressor REST is a critical regulator of the neurosecretory phenotype. J Neurochem. 98(6):1828-40, 2006. PMID: 16945103
- Nagel AC, Krejci A, Tenin G, Bravo-Patino A, Bray S, Maier D, Preiss A. : Hairless-mediated repression of notch target genes requires the combined activity of Groucho and CtBP corepressors. Mol Cell Biol. 25(23):10433-41, 2005. PMID: 16287856
- Jakubik J, Krejci A, Dolezal V: Asparagine, valine, and threonine in the third extracellular loop of muscarinic receptor have essential roles in the positive cooperativity of strychnine-like allosteric modulators. J Pharmacol Exp Ther. 313(2):688-96, 2005.
- Krejci A, Bruce AW, Tucek S, Dolezal V, Buckley NJ: Multiple promoters drive tissue specific expression of the human M2 muscarinic acetylcholine receptor gene. J Neurochem. 91(1):88-98, 2004.
- Tucek S, Krejci A, Lysikova M, Jakubik J, Dolezal V: Roles of external loops of muscarinic receptors in interactions between N-methylscopolamine and allosteric modulators. In Cholinergic mechanisms: Function and disfunction. Ed. I. Silman, 71-76, 2004.
- Krejci A, Michal P, Jakubik J, Ricny J, Dolezal V: Regulation of signal transduction at M2 muscarinic receptor. Physiol. Res., 53, suppl.1:S131-40, 2004.
- Krejci A, Tucek S: Quantitation of mRNAs for M1 to M5 subtypes of muscarinic receptors in rat heart and brain cortex. Mol Pharmacol. 61(6):1267-72, 2002.
- Krejci A, Tucek S: Changes of cooperativity between N-methylscopolamine and allosteric modulator alcuronium and gallamine induced by mutations of external loops of muscarinic M3 receptors. Mol. Pharmacol. 60 : 761-767, 2001.
ORCID number: 0000-0001-7589-4035