7 PhD positions

Seven PhD positions in Molecular Biology & Genetics for 4 years 2020-2024 - apply by 15 Apr 2020

Available research projects

> Doležel: Circadian clock in Drosophila melanogaster

> Doležel: Evolution & phylogeography of the linden bug, Pyrrhocoris apterus

> Gahura: Structure, function and biogenesis of divergent trypanosomal mitoribosomes

> Lukeš: Functional genomics of trypanosomes and their endosymbionts

> Nguyen: Neo-sex chromosome evolution in Lepidoptera

> Paris: Essential tRNA modifications in trypanosomatid parasites

> Zíková: The role of Trypanosoma brucei ATP synthase dimers to shape cristae and affect mitochondrial biogenesis


> PhD study for 4 years 2020-2024 (possible start in Aug/Sep 2020),

> professional career development in science & soft skills,

> participation in cutting-edge research projects,

> access to the state-of-the-art research infrastructure & equipment,

> supervision by outstanding mentors, focus on activity, creativity & innovativeness,

> international collaboration & visibility, international short-term research visits,

> English speaking, stimulating and friendly environment,

> PhD fellowship & part-time job employment on research grants,

> administration support with relocation & settlement in the Czech Republic,

> participation in annual PhD retreat,

> meals allowance, full health insurance, student benefits,

> work-life balance in a middle-sized university city offering options for outdoor, sport & cultural activities.


> MSc in Molecular Biology, Genetics or similar,

> proficiency in English – oral and written,

> openness to learn new techniques, active attitude to problem-solving,

> ability to work in a team,

> independent thinking & passion for science.

Please submit your structured CV including list of publications, motivation letter, name & contacts of two academic referees via e-mail to jobs@prf.jcu.cz by Wed 15 Apr 2020. Interviews with selected applicants will be held in April 2020.

Info about PhD studies in general: www.prf.jcu.cz/en/kmb/for-students/doctoral-studies.html

Details about the individual projects:

David Doležel Group: Circadian clock in Drosophila melanogaster

Contact: dolezel@entu.cas.cz, www

The internal time-keeping device, circadian clock, is remarkably conserved across animals. Unparalleled genetic tools established the fruit fly Drosophila as an excellent model to elucidate its underlying molecular mechanisms. PhD candidate will use wide palette of molecular and genetic tools including CRISPR/Cas9, transgenesis, mutagenesis, gene cloning, and in silico analysis to decipher principles underlying circadian clocks.

David Doležel Group: Evolution & phylogeography of the linden bug, Pyrrhocoris apterus

Contact: dolezel@entu.cas.cz, www

Pyrrhocoris apterus is an emerging insect model used to elucidate molecular and genetic basis of neuroendocrine signalling, seasonality, circadian clocks and diapause. PhD candidate will perform phylogenetic analyses using our genomic and transcriptomic data and perform thorough reconstruction of P. apterus phylogeography relying on our large field-line collections. Training in experimental techniques and verification of in silico obtained data by reverse genetics will be possible as well.

Ondřej Gahura Group: Structure, function and biogenesis of divergent trypanosomal mitoribosomes

Contact: gahura@paru.cas.cz, www

Ribosomes, highly conserved machines fundamental for all life forms, were recently shown to undergo unexpected diversification in endosymbiotic organelles with vestigial genomes. The most divergent ribosomes are found in mitochondria of the pathogenic protist Trypanosoma brucei. We complement the wealth of cryoEM structural data on trypanosomal mitoribosomes and their precursors with functional approaches to get insight into unique features of the mitochondrial protein synthesis machinery of the parasite.

Julius Lukeš Group: Functional genomics of trypanosomes and their endosymbionts

Contact: jula@paru.cas.cz, www

Our laboratory studies various aspects of gene functions in trypanosomes, primarily on human infective Trypanosoma brucei but also related flagellates. We focus on proteins functionally associated with the mitochondrion and dissect their functions with a wide range of methods. Moreover, we also study the relationship of endosymbiotic bacteria with their trypanosomatid hosts.

Petr Nguyen Group: Neo-sex chromosome evolution in Lepidoptera

Contact: petr.nguyen@prf.jcu.cz, www

Unlike in other taxa with female heterogamety (WZ/ZZ), neo-sex chromosomes are common in moths and butterflies (Lepidoptera). The project aims at investigating drivers of lepidopteran neo-sex chromosome evolution and a role of sex chromosome turnover in species divergence. It will encompass generating genomic resources, expression profiling, cytogenetic and population genetic analyses in several non-model taxa such as ghost moth chromosome races and butterflies of the tribe Danaini.

Zdeněk Paris Group: Essential tRNA modifications in trypanosomatid parasites

Contact: parda@paru.cas.cz, www

We study various aspects of RNA biology of protozoan parasite Trypanosoma brucei and related flagellates. In these early evolved unicellular organisms most genes are post-transcriptionally regulated. Consequently, posttranscriptional processing of RNA is of a great importance in order to regulate the complex life cycles of these pathogens. Research in our group focuses on processes such as tRNA maturation, nuclear export/import and tRNA modifications in trypanosomes. Our long term goal is the identification of unique RNA metabolism mechanisms. We believe this will help to identify new drug targets to combat diseases caused by trypanosomatid parasites.

Alena Zíková Group: The role of Trypanosoma brucei ATP synthase dimers to shape cristae and affect mitochondrial biogenesis

Contact: azikova@paru.cas.cz, www

We focus on the unique bioenergetic properties of the lethal human parasite, Trypanosoma brucei. The singular mitochondrion of this genetically tractable model organism undergoes dramatic structural and metabolic remodelling while responding to the different nutrients found in the various environments it encounters throughout its life cycle. Current projects in the lab include the following: 1) how mitochondrion metabolism adapts to various carbon sources; 2) the role of ATP synthase dimers to shape cristae and affect mitochondrial biogenesis; 3) how the mitochondrion acts as a signalling organelle during cellular differentiation.