RNDr. Ján Štěrba, Ph.D.


 

ORCID iD iconhttps://orcid.org/0000-0003-1005-1787

 

Projects

2006: Glycosylation of tick-borne encephalitis virus envelope protein and its influence on virus virulence – Grant Agency of the University of South Bohemia.

2007: Glycosylation of membrane proteins of Lyme borreliosis spirochetes – Grant Agency of the University of South Bohemia.

2009-2011: Glycoprotein analysis of Ixodes ricinus salivary glands – Grant Agency of the Academy of Sciences of the Czech Republic (PI: Dr. Marie Vancová, Institute of Parasitology, BC ASCR, České Budějovice, co-PI: Ján Štěrba, Faculty of Science, University of South Bohemia, České Budějovice).

2017-2019: sub-project 01-01 Verification of tick-borne encephalitis virus inhibition by some natural compounds (part of a TAČR Gama project TG03010027) (PI of the sub-project – Ján Štěrba);

2018-2019: sub-project 02-13 Preparation of durable nanostructured surfaces with immobilized bioactive molecules (part of a TAČR Gama project TG03010027) (PI of the sub-project – Ján Štěrba)

2018-2021: Development of technologies for early detection of tick borne encephalitis, based on changes in gene expression and protein production in infected antigen-presenting cells.

 participation on several projects of Grant Agency of Academy of Sciecens of the Czech Republic, Grant Agency of the Czech Republic, Ministry of Education, Youth and Sports; Research infrastructure C4SYS, National Centre of Competence MATCA

Teaching

Biochemické praktikum pro Biologii a ŽP (UCH/051) - winter term, in Czech

 Basic laboratory course in Biochemistry for students of study programmes not oriented to Chemistry, Molecular Biology, and Medicinal Chemistry.

 

Praktikum z biochemie (UCH/049) - winter term, in Czech

Basic laboratory course in Biochemistry for students of study programmes oriented to Chemistry, Molecular Biology, and Medicinal Chemistry.

 

Biochemistry Laboratory 1 (UCH/758) - winter term, in English

Basic laboratory course in Biochemistry for students of the Biological Chemistry study programme.

 

Pokročilé praktikum z biochemie (UCH/050) - summer term, in Czech

Advanced laboratory course in Biochemistry for students of the Chemistry study programme.

Biochemistry Laboratory 2 (UCH/642) - summer term, in English

Advanced laboratory course in Biochemistry for students of the Biological Chemistry study programme.

Farmakologie a toxikologie (KME/748) - winter term, in Czech

Course offered especially for the students of the study programmes Biomedicinal Laboratory technique and Clinical Biology. Basics in pharmacology, mode of action of drugs on molecular level, basics in pharmacogenetics,clinical trials, basics in toxicology and xenobiochemistry, action of toxic substances on molecular level. Lab. course: pharmacogenetics in practice - cytochromes and aldehyde- and alcohodehydrogenase.

Úvod do toxikologie (UCH/112) - winter term, in Czech

Course offered especially for the students of the Chemistry study programme. Basics in toxicology, action of toxic substances on molecular leve, reactions in detoxification - Phase I and Phase reaction in xenobiochemistry. Lab. course: toxicogenetics in practice - cytochromes and aldehyde- and alcohodehydrogenase.

Xenobiochemistry and Toxicology (UCH/052) - summer term, in English

Course offered especially for the students of the Biological Chemistry study programme. Basics in toxicology, action of toxic substances on molecular leve, reactions in detoxification - Phase I and Phase reaction in xenobiochemistry. Lab. course: toxicogenetics in practice - cytochromes and aldehyde- and alcohodehydrogenase.

Supervised BSc. and MSc. theses

Published scientific papers

Růžek D., Kopecký J., Štěrba J., Golovchenko M., Rudenko N., Grubhoffer L.: Non-virulent strains of TBE virus circulating in the Czech Republic. Journal of Clinical Virology 36 (suppl. 2): S41, 2006 (P117).

Růžek D., Štěrba J., Kopecký J., Grubhoffer L.: The supposedly attenuated Hy-HK variant of highly virulent Hypr strain of tick-borne encephalitis virus is obviously a stain of Langat virus. Acta Virologica 50(4), 227-278, 2006.

Štěrba J., Vancová M., Rudenko N., Golovchenko M., Tremblay T.-L., Kelly J.F., MacKenzie R.C., Logan S.M., Grubhoffer L.: Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated. Journal of Bacteriology 190: 2619-2623, 2008.

Man P., Kovář V., Štěrba J., Strohalm M., Kavan D., Kopáček P., Grubhoffer L., Havlíček V.: Deciphering Dorin M glycosylation by mass spectrometry. European Journal of Mass Spectrometry 14: 345-354, 2008.

Štěrba J., Dupejová J., Fišer M., Golovchenko M., Rudenko N., Grubhoffer L.: Identification and characterisation of lectins in several tick species. FEBS Journal, 276 (Suppl. 1): 88, 2009.

Grubhoffer L., Hajdušek O., Vancová M., Štěrba J., Rudenko N.: Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans. FEBS Journal, 276 (Suppl. 1): 141, 2009.

Dupejova J., Sterba J., Vancova M., Grubhoffer L.: Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization. Parasites&Vectors, 4:4, 2011.

Sterba J., Dupejova J., Fiser M., Vancova M., Grubhoffer L.: Fibrinogen-related proteins in ixodid ticks. Parasites&Vectors, 4:127, 2011.

Vancova M., Sterba J., Dupejova J., Simonova Z., Nebesarova J., Novotny M.V., Grubhoffer L.: Uptake and incorporation of sialic acid by the tick Ixodes ricinus. Journal of Insect Physiology, 2012, 58, 1277-1287.

Grubhoffer L., Rudenko N., Vancova M., Golovchenko M., Sterba J.: Circulatory system and hemolymph. In: Biology of ticks. Vol. 1. Daniel E. Sonenshine (Editor), R. Michael Roe (Editor). Oxford University Press Inc; 2013, 2nd Revised edition. pp. 258-286.

Sterba J., Vancova M., Sterbova J., Bell-Sakyi L., Grubhoffer L.: The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick. Carbohydrate Research, 2014, 389, 93-99.

Villar M, Ayllón N, Kocan KM, Bonzón-Kulichenko E, Alberdi P, Blouin EF, Weisheit S, Mateos-Hernández L, Cabezas-Cruz A, Bell-Sakyi L, Vancová M, Bílý T, Meyer DF, Sterba J, Contreras M, Rudenko N, Grubhoffer L, Vázquez J, de la Fuente J. Identification and characterization of Anaplasma phagocytophilum proteins involved in infection of the tick vector, Ixodes scapularis. PLoS One. 2015, 10, e0137237. 10.1371/journal.pone.0137237

Hönig V, Svec P, Halas P, Vavruskova Z, Tykalova H, Kilian P, Vetiskova V, Dornakova V, Sterbova J, Simonova Z, Erhart J, Sterba J, Golovchenko M, Rudenko N, Grubhoffer L.: Ticks and tick-borne pathogens in South Bohemia (Czech Republic) - Spatial variability in Ixodes ricinus abundance, Borrelia burgdorferi and tick-borne encephalitis virus prevalence. Ticks Tick Borne Dis, 2015, 6, 559-67. 10.1016/j.ttbdis.2015.04.010

Černý J, Selinger M, Palus M, Vavrušková Z, Tykalová H, Bell-Sakyi L, Štěrba J, Grubhoffer L, Růžek D.: Expression of a second open reading frame present in the genome of tick-borne encephalitis virus strain Neudoerfl is not detectable in infected cells. Virus Genes 2016, 1-8. 10.1007/s11262-015-1273-y

Dzyuba V, Słowińska M, Cosson J, Boryshpolets S, Štĕrba J, Rodina M, Linhart O, Dzyuba B.: Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation. Fish Physiol Biochem, 2016, 42, 1755-66. 10.1007/s10695-016-0255-x

Shaliutina-Kolešová A, Kotas P, Štěrba J, Rodina M, Dzyuba B, Cosson J, Linhart O.: Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss). Mol Reprod Develop, 2016, 83: 968-982. 10.1002/mrd.22737.

Kratochvíl J, Kahoun D, Štěrba J, Langhansová H, Lieskovská J, Fojtíková P, Hanuš J, Kousal J, Kylián O, Straňák V. Plasma polymerized C:H:N:O thin films for controlled release of antibiotic substances. Plasma Process Polym. 2018; 15:e1700160. 10.1002/ppap.201700160

Xin M, Shaliutina-Kolesova A, Sterba J, Konik P, Boryshpolets S, Rodina M, Li P, Nian R, Linhart O. Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome. Animal Reproduction Science, 2018, 192, 280-9. 10.1016/j.anireprosci.2018.03.025

Kratochvíl J, Štěrba J, Lieskovská J, Langhansová H, Kuzminova A, Khalakhan I, Kylián O, Straňák V. Antibacterial effect of Cu/C:F nanocomposites deposited on PEEK substrates, Materials Letters, 2018, 230, 69-99. doi: https://doi.org/10.1016/j.matlet.2018.07.082

Xin, M., Sterba, J., Shaliutina-Kolesova, A. Dzyuba B, Lieskovska J, Boryshpolets S, Siddique MAM, Kholodnyy V, Lebeda I, Linhart O. Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation. Fish Physiol Biochem, 2018, 44: 1527-33. https://doi.org/10.1007/s10695-018-0538-5

 Dominik Schwarz, Amelie K.J. Schoenenwald, Jörg Dörrstein, Jan Sterba, David Kahoun, Pavla Fojtíková, Josef Vilímek, Doris Schieder, Cordt Zollfrank, Volker Sieber, Biosynthesis of poly-3-hydroxybutyrate from grass silage by a two-stage fermentation process based on an integrated biorefinery concept, Bioresource Technology, 2018, 269, 237-245. https://doi.org/10.1016/j.biortech.2018.08.064

Vechtova P, Sterbova J., Sterba J, Vancova M, Rego ROM, Selinger M, Strnad M, Golovchenko M, Rudenko N, Grubhoffer L, A bite so sweet: the glycobiology interface of tick-host-pathogen interactions. Parasit Vectors, 2018, 11:594. https://doi.org/10.1186/s13071-018-3062-7

Dzyuba V, Sampels S, Ninhaus-Silveira A, Kahanec M, Veríssimo-Silveira R, Rodina M, Cosson J, Boryshpolets J, Selinger M, Sterba J, Dzyuba B, Sperm motility and lipid composition in internally fertilizing ocellate river stingray Potamotrygon motoro. Theriogenology, 2019, 130: 26-35. https://doi.org/10.1016/j.theriogenology.2019.02.029.

Vaidulych M, Shelemin A, Hanuš J, Khalakhan I, Krakovsky I, Kočová P, Mašková H, Kratochvíl J, Pleskunov P, Štěrba J, Kylián O, Choukourov A, Biederman H, Superwettable antibacterial textiles for versatile oil/water separation. Plasma Process Polym. 2019;e1900003. http://dx.doi.org/10.1002/ppap.201900003

Loginov DS, Loginova YF, Dycka F, Böttinger K, Vechtova P, Sterba J. Tissue-specific signatures in tick cell line MS profiles. Parasit Vectors, 2019, 12:212. https://doi.org/10.1186/s13071-019-3460-5.

Xin M, Niksirat H, Shaliutina-Kolesova A, Siddique MAM, Sterba J, Boryshpolets S, Linhart O. Molecular and subcellular cryoinjury of fish spermatozoa and approaches to improve cryopreservation. Reviews in Aquaculture, 2020, 12, 909–924, https://doi.org/10.1111/raq.12355

Kratochvíl J, Kahoun D, Kylián O, Štěrba J, Kretková T, Kousal J, Hanuš J, Vaclová J, Prysiazhnyi V, Sezemský P, Fojtíková P, Lieskovská J, Langhansová H, Krakovský I, Straňák V. Nitrogen enriched C:H:N:O thin films for improved antibiotics doping. Applied Surface Science, 2019, 494, 301-308. ISSN 0169-4332, https://doi.org/10.1016/j.apsusc.2019.07.135

Selinger M, Tykalová H, Štěrba J, Věchtová P, Vavrušková Z, Lieskovská J, Kohl A, Schnettler E, GrubhofferL. Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. PLoS Negl Trop Dis, 2019, 13:e0007745. https://doi.org/10.1371/journal.pntd.0007745

  Xin, M.; Vechtova, P.; Shaliutina-Kolesova, A.; Fussy, Z.; Loginov, D.; Dzyuba, B.; Linhart, O.; Boryshpolets, S.; Rodina, M.; Li, P.; Loginova, Y.; Sterba, J. Transferrin Identification in Sterlet (Acipenser ruthenus) Reproductive System. Animals 2019, 9, 753. https://doi.org/10.3390/ani9100753

Prysiazhnyi V., Dycka F. Kratochvil J., Sterba, J. Stranak V. Gas-aggregated Ag nanoparticles for detection of small molecules using LDI MS. Analytical and Bioanalytical Chemistry. 2019, 412, 1037-1047. doi:10.1007/s00216-019-02329-5

 Kotsarenko, P. Vechtova, Z. Hammerova, N. Langova, L. Malinovska, M. Wimmerova, J. Sterba, and L. Grubhoffer. Newly identified DNA methyltransferases of Ixodes ricinus ticks. Ticks and tick-borne diseases, 2020, 11:101348. 10.1016/j.ttbdis.2019.101348

Rusanov AR, Stepanov AA, Zgoda V, Kaysheva A, Selinger M, Maskova H, Lginov D, Sterba J, Grubhoffer L, Luzgina NG. Proteome dataset of mouse macrophage cell line infected with tick-borne encephalitis virus. Data in Brief. 2020, 28:105029.  https://doi.org/10.1016/j.dib.2019.105029

Loginov DS, Böttinger K, Loginova YF, Dycka F, Vechtova P, Sterba J. Biotyping of IRE/CTVM19 tick cell line infected by tick-borne encephalitis virus. Ticks Tick Borne Dis. 2020;11(4):101420. doi:10.1016/j.ttbdis.2020.101420

Vaidulych M, Pleskunov P, Kratochvíl J, Mašková H, Kočová P, Nikitin D, Hanuš J, Kylián O, Štěrba J, Biederman H, Choukourov, A. Convex vs concave surface nano-curvature of Ta2O5 thin films for tailoring the osteoblast adhesion, Surface and Coatings Technology, 2020, 393, 125805. https://doi.org/10.1016/j.surfcoat.2020.125805.

Vechtova P, Fussy Z, Cegan R, Sterba J, Erhart J, Benes V, Grubhoffer L. Catalogue of stage-specific transcripts in Ixodes ricinus and their potential functions during the tick life-cycle. Parasites Vectors 13, 311 (2020). https://doi.org/10.1186/s13071-020-04173-4

Kotsarenko K, Vechtova P, Lieskovska J, Füssy Z, Cabral-de-Mello D, Rego R, Alberdi P, Collins M, Bell-Sakyi L, Sterba J, Grubhoffer L. Karyotype changes in long-term cultured tick cell lines, Scientific Reports  2020, 10, 13443. https://doi.org/10.1038/s41598-020-70330-5

Jiří Kratochvíl, Vadym Prysiazhnyi, Filip Dyčka, Ondřej Kylián, Peter Kúš, Petr Sezemský, Ján Štěrba, Vítězslav Straňák. Gas aggregated Ag nanoparticles as the inorganic matrix for laser desorption/ionization mass spectrometry. Applied Surface Science. 2021, 541, 148469. https://doi.org/10.1016/j.apsusc.2020.148469

Cheng Y., Vechtova P., Fussy Z., Sterba J., Linhartová Z., Rodina M., Tučková V., Gela D., Samarin A.M., Lebeda I., Xin M., Zhang S., Rahi D., Linhart O. Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp Cyprinus carpio. Int. J. Mol. Sci. 2021, 22, 5925. https://doi.org/10.3390/ijms22115925

Rusanov AL, Kozhin PM, Tikhonova OV, Zgoda VG, Loginov DS, Chlastáková A, Selinger M, Sterba J, Grubhoffer L, Luzgina NG. Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages. International Journal of Molecular Sciences. 2021; 22(12):6323. https://doi.org/10.3390/ijms22126323

Azinfar A, Nueber S, Vancova M, Sterba J, Stranak V, Helm C. Self-patterning polyelectrolyte multilayer films: influence of deposition steps and drying in vacuum. Langmuir. 2021; Accepted. https://doi.org/10.1021/acs.langmuir.1c01409

 Hejduk L, Rathner P, Strnad M, Grubhoffer L, Sterba J, Rego ROM, Müller N, Rathner A. Resonance assignment and secondary structure of DbpA protein from the European species, Borrelia afzelii. Biomol NMR Assign 2021, 15(2), 415-420. https://doi.org/10.1007/s12104-021-10039-2

Dadras H, Policar T, Loginov D, Boryshpoletsa S, Loginova Y, Sampels S, Sotnikov A, Kholodnyy V, Sterba J, Xin M, Dzyuba B, Relationship of motility activation to lipid composition, protein profile, and swelling rate of burbot Lota lota spermatozoon following change of temperature and osmolality. Front Mar Sci, 2021 8, 1551. https://doi.org/10.3389/fmars.2021.736087

Forinová, Michala; Pilipenco, Alina; Víšová, Ivana; Lynn Jr., N. Scott; Dostalek, Jakub; Mašková, Hana; Hönig, Václav; Palus, Martin; Selinger, Martin; Kočová, Pavlína ; Dyčka, Filip; Štěrba, Jan; Houska, Milan; Vrabcová, Markéta; Horák, Petr; Anthi, Judita; Tung, Chao-Ping; Yu, Chung-Ming; Chen, Chi-Yung ; Huang, Yu-Chuan; Tsai, Pei-Hsun; Lin, Szu-Yu; Hsu, Hung-Ju; Yang, An-Suei; Dejneka, Alexandr; Vaisocherová - Lísalová, Hana. Functionalized Terpolymer Brush-Based Biointerface with Improved Antifouling Properties for Ultra-Sensitive Direct Detection of Virus in Crude Clinical Samples. ACS Applied Materials & Interfaces, 2021, accepted.

Presentations at conferences:

2014

13th Bratislava Symposium on Saccharides, Smolenice, Slovakia, lecture: „The origin of sialic acid in adult ticks and its localization“; poster: „Localization and identification of fucosylated glycoproteins in Ixodes ricinus ticks using bioorthogonal chemistry“.

4th Visegrad Symposium on Structural Systems Biology, Nové Hrady, Czech Republic, lecture: „Glycosylation – from proteins to an organism“.

2013

17th European Carbohydrate Symposium (EuroCarb 17), Tel-Aviv, Israel, two posters: „Majority of sialylated glycoproteins in adult ticks originates in the host, not the tick“,„Fucosylation in Ixodes ricinus tick: localization and purification of fucosylated glycoproteins using bioorthogonal chemistry“.

2012

XXIII. Biochemický sjezd, Brno, Czech Republic, lecture: „Advances in tick glycobiology“; poster: „All that glitters is not gold … or glycan. (Detection of glycans in Ixodes ricinus tick cell cultures and tissues using bioorthogonal chemistry)“.

2011

XI. Sigma-Aldrich konference mladých biochemiků a molekulárních biologů, Žďár n. Sázavou, ČR: lecture “N-glycans in saliva and salivary glands of the tick Ixodes ricinus”;

Ticks and Tick-borne Pathogens Conference 7, Zaragoza, Spain: “Ornithodoros moubata cell culture producing the tick lectin, Dorin M”, Sterba J., Dupejova J., Vancova M., Palus M., Bell-Sakyi L., Grubhoffer L.; “Characterization of the hemelipoglycoprotein from the tick, Dermacentor marginatus”, Dupejova J., Sterba J., Vancova M., Grubhoffer L.; “Mass spectrometric identification and electron microscopy localization of sialic acids in Ixodes ricinus salivary glands”, Vancova M., Sterba J., Dupejova J., Nebesarova J., Novotny M.V., Grubhoffer L.; “Glycobiology of ticks” Grubhoffer L., Dupejova J., Vancova M., Fiser M., Novotny M.V., Sterba J.

XV. Setkání biochemiků a molekulárních biologů, Brno, ČR: “Core-fucosylation in Ixodes ricinus ticks: Life-stage- and structure-specific detection”, Dupejova J., Sterba J., Vancova M., Novotny M.V., Grubhoffer L.

2010

25th international symposium on Microscale bioseparations MSB 2010, 21.-25.3.2010: Sterba J. Vancova M., Alley WR, Mann BF, Bucinska L, Dupejova J, Grubhoffer L, Novotny MV P195: Analysis of minute volumes of tick saliva samples using MALDI TOF/TOF mass spectrometry, p.158

XIV. Setkání biochemiků a molekulárních biologů, 20.-21.4.2010: Štěrba J., Vancová M., Alley WR Jr., Mann BF, Bučinská L, Dupejová J, Grubhoffer L, Novotný MV N-glycans from the saliva and salivary glands of the tick Ixodes ricinus, p.39-40

2009

Curent chemistry and biochemistry of carbohydrates - Cukrblik 2009, 16.4.2009: Štěrba J., Man P., Kovář V., Dupejová J., Fišer M., Strohalm M., Kavan D., Kopáček P., Vancová M., Havlíček V., Grubhoffer L. Fibrinogen-related proteins/lectins in ticks, p. 17; Vancová M., Bučinská L., Štěrba J., Nebesářová J., Grubhoffer L. The presence of sialic acid in the salivary glands of the tick Ixodes ricinus, p.35

2008

XXI. Biochemický sjezd: Grubhoffer L, Hajdušek O, Vancová M, Štěrba J, Rudenko N O97 Glykobiochemie klíšťat – přenašečů závažných onemocnění: sacharidy vázající proteiny a glykany, p.187; Vancová M, Bučinská L, Štěrba J, Grubhoffer L P167 The presence of N-acetylneuraminic acid modified protein in salivary glands of ticks Ixodes ricinus, p.192; Dupejová J, Śtěrba J, Vancová M, Kovář V, Grubhoffer L P115 Fibrinogen-related proteins (elctins) in tick Dermacentor marginatus hemolymph, p.136

2007

Structure and stability of biomacromolecules SSB 2007, 5.-8.9.2007: Štěrba J, Vancová M, Rudenko N, Golovchenko M, Kelly JF, MacKenzie RM, Logan SM, Grubhoffer L PO35 Structural characterization of outer surface (Osp) and flagellin (Fla) proteins from Borrelia burgdorferi, p.121

8th Multinational Congress on Microscopy, 17.-21.6.2007: Vancová M, Nebesářová J, Vaněček J, Štěrba J, Fránová J LP-58 Observation of Flagella and Viruses in Low-Voltage Transmission Electron Microscope, p.545

2006

XX. biochemický zjazd, 12.-16.9.2006: Štěrba J, Vancová M, Rudenko N, Golovchenko M, Kelly J, Logan S, MacKenzie R, Grubhoffer L PO9.13 Glycosylation in Lyme borreliosis spirochetes, p.396