Although obesity and obesity-associated diseases are the most frequently occurring diseases worldwide, adequate medical treatment is missing. Obese adipose tissue contains hypertrophic adipocytes surrounded by macrophages forming crown-like structures. Although macrophages primarily take up lipids to protect the organism from the negative impact of ectopic lipid deposits, the lipid accumulation affects their phenotypic response substantially so that lipid laden macrophages produce factors impairing insulin signaling. We discovered for the first time the presence of lipid-laden macrophages forming the crown-like structures during adipose tissue remodeling in Drosophila. Therefore, we want to implement this model in order to study the response of macrophages on lipid internalization as well as the impact of the adopted phenotype on the systemic physiology of the individual. By using a combination of Drosophila genetic tools with molecular biology techniques, we may enhance the understanding of the mechanisms of obesity-associated diseases and fully establish a novel experimental system.
Bajgar Adam Mgr. et Mgr. Ph.D.
Katedra molekulární biologie a genetiky