Insulin resistance (IR) is mostly considered a pathological condition. In humans, IR is indeed associated with a number of health problems, but we still do not have a good understanding of why it is developing. Its occurrence in many different organisms and in many different contexts indicates that there must be physiological reasons for such a metabolic program. The aim of the proposed project is to study the physiological role of IR in acute immune response and its association with pathological consequences in Drosophila. Our preliminary results suggest that Impl2 could be used by immune cells to inhibit insulin signaling during the immune response, which we could use to test the importance of IR for the effectiveness of the immune response. Since Impl2 was identified as a mediator of cancer-induced wasting, this investigation could combine the physiological role of IR with pathological consequences as a wasting during chronic infection or cancer. We also plan to analyze how insect cells acquire nutrients in the state of IR to broaden the evolutionary view of this phenomenon. 1. Determine whether insulin resistance is important for an effective immune response and if immune cell-specific release of Impl2 mediates insulin resistance. 2. Investigate how insect immune cells obtain nutrients in an insulin resistance state. Do they use trehalose to be privileged in energy use?
Doležal Tomáš Mgr., Ph.D.
Katedra molekulární biologie a genetiky