Faculty of Science, University of South Bohemia
Branišovská 31, 37005 České Budějovice
building B, ground floor, door number 193
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Tel. +420 387772229
ORCID ID: 0000-0001-5217-4465
doc. Mgr. Tomáš Doležal, Ph.D.
Head of the Laboratory of Molecular Integrative Physiology in Drosophila (DrosoPhyLab)
A Trends in Biochemical Sciences spotlight
Our recent work (Nedbalova et al.) was highlighted in Trends in Biochemical Sciences, read a publication by Tina Mukherjee.
Research Highlight in Science journal
Excited to see the work of our Ph.D. candidate Pavla Nedbalova featured in a Science dispatch! SAM metabolism and adenosine recycling shown to be central to systemic regulation & immunity.
Our findings in eLife digest
Read more about the finding from our lab, that adenosine may be a central player in the mechanism that allows cells to measure their activity and require nutrients.
ScienceZoom University of South Bohemia
A new take on a familiar molecule: using adenosine, the cell asks for more energy. According to South Bohemian scientists, this is fundamentally related to how our immunity works (article in Czech).
Pavla Nedbalová published her first-author paper in eLife
How does a cell measure its current activity in order to ask its neighborhood for an adequate supply of nutrients? Pavla Nedbalova have found a possible mechanism, published in the journal eLife.
New publication in PLOS Biology
A new publication by Michalina Kazek and Lenka Chodáková shows how important it is for organisms to set the balance between the strength of the immune response and protecting themselves from the toxic effects of their own immune response.
The Good and Bad of Insulin Resistance
A new publication by Ellen McMullen in Cell Communication and Signaling is showing that acute insulin resistance is a beneficial metabolic program that is required for an effective immune response, only becoming pathology in the chronic state.
How to eliminate pathogen without killing oneself?
What we know about the metabolism of insect immune response, while placing it in the context of possible implications for host physiology, and highlighting open questions related to the metabolism. Perspective article by Tomáš Doležal.
Current possitions
Group leader | ||
Member of the Scientific Board | Faculty of Science, University of South Bohemia, České Budějovice | |
Chair of the Programme Board | ||
Guarantor of the doctoral study programme | ||
Guarantor of the master study programme | Experimental biology - Molecular and Cell Biology and Genetics |
Previous possitions
Head of the Department of Molecular Biology and Genetics | Faculty of Science, University of South Bohemia, České Budějovice | |
Visiting assistant professor | Stanford University School of Medicine, USA | |
Laboratory of Prof. Peter Bryant | University of California, Irvine, USA | |
Laboratory of Dr. Michal Žurovec | Institute of Entomology ASCR, České Budějovice | |
Laboratory of Prof. Peter Bryant | University of California, Irvine, USA | |
Laboratory of Molecular Genetics and DNA Diagnosis | 1st Medical Faculty, Charles University, Prague |
Education
Habilitation (doc.) Molecular and Cell Biology and Genetics | Faculty of Science, University of South Bohemia, České Budějovice | |
Ph.D., Molecular and Developmental Biology and Genetics | Faculty of Biology, University of South Bohemia, České Budějovice | |
MSc., Genetics, Molecular Biology and Immunology | Faculty of Science, Charles University, Prague |
Awards
Rheos Short Talk Award | 2nd Metabolism in Health and Disease Conference 2022, Cancun, Mexico | |
Prize of the Learned Society of the Czech Republic | in the category of Researcher of the Year 2017 for the validation of the new concept of selfish immunity | |
cena Barbary Burgess | for excellence in postdoctoral research at University of California, Irvine, USA. Project: Role of ADGF-A gene in Drosophila |
International scientific activity
visiting assistant professor | Stanford University School of Medicine, USA (1.5 yrs) | |
postdoctoral fellowship, Prof. Peter Bryant's lab | University of California, Irvine, USA |
Research projects (principal investigator)
GAČR 24-10313S | Koordinace vývoje a imunitní obranyschopnosti systémovým hormonálním signálem (co-funded together with prof. RNDr. Marek Jindra, CSc.) | |
GAČR 20-09103S | Molekulární mechanismy privilegovaného přístupu aktivovaných imunitních buněk k energii u drozofily | |
GAČR 17-16406S | Vznik adenozinu jako signálu sobecké imunity | |
Marie Curie International Outgoing Fellowship 298186 | Extracellular adenosine role in energetic metabolism during immune response | |
GAČR P305/12/0115 | Model drozofily pro studium účinků extracelulárního adenozinu na mechanismy rezistence a tolerance k patogenům | |
GAČR 204/09/1463 | Extracelulární adenozin jako stresový hormon v drozofile | |
GAČR 301/07/0814 | Zkoumání úlohy kasein kinázy Iepsilon pro vznik rakoviny prsu | |
GAAV KJB501410602 | Úlohy extracelulárního adenozinu při krvetvorbě a vrozené imunitě za použití drozofily jako modelu pro studium těchto úloh in vivo |
Scientific projects (hosting lab, mentor)
EMBO Postdoctoral Fellowship | Dr. Ellen McMullen | |
MŠMT OPVVV, 02-20-079 MSCA IF IV | Dr. Michaela Fencková | |
Marie Skłodowska-Curie grant | Dr. Michalina Kazek |
Development projects (principal investigator)
Operational Programme Education for Competitiveness (OP VK) | Molecularization of biological disciplines at the Faculty of Science, University of South Bohemia, České Budějovice | |
FRVŠ | Establishment of a joint laboratory for practical teaching of organic chemistry and molecular biology |
Invited lectures
Metabolism of privileged immune cells | Drosophila Blood Cell Biology Symposium, Strasbourg, France | |
Why is our immune system selfish? | Kužela seminar, Comenius University in Bratislava | |
Molecular mechanisms connecting activation of immune cells with metabolic changes at systemic level | Immunity and Hematopoeisis Workshop, European Drosophila Research Conference 2017, London | |
Energy privilege of activated immune system | Lecture for the Czech Immunological Society, Prague | |
What did we learn from fruit flies about a selfish behavior of immune system? | Lecture for the Learned Society of the Czech Republic, Prague | |
Adenosine as a signal of selfsh immune system - fruit fly model | ESID Eastern Spring School in Primary Immunodeficiencies, Třeboň |
Oral presentations at international conferences
The origin of adenosine as a systemic metabolic regulator in privileged immune cells | 2nd Metabolism in Health and Disease Conference, Cancun, Mexico | |
Molecular regulations of metabolism during immune response in Drosophila. | T. Dolezal, A. Bajgar, G. Krejcova, P. Nedbalova - Eighth International Symposium on Molecular Insect Science (Elsevier) 7-10 July 2019 Sitges, Spain | |
Extracellular adenosine regulates complex host-pathogen interactions through the energy release for the immune response | T. Dolezal, A. Bajgar, K. Kucerova, L. Jonatova, DS Schneider - 55th Annual Drosophila Research Conference, 26.–30.3. 2014, San Diego, USA | |
The discs overgrown (dco) mutant - a fly model for the role of Casein Kinase Iε in breast cancer | T. Dolezal, J. Matulova, K. Kucerova, E. Kubala, PJ Bryant - 21st European Drosophila Research Conference, Nice, France | |
Adenosine Deaminase-related Growth Factors (ADGFs) and extracellular adenosine mediate the juvenile hormone response in Drosophila | Great Barrier Reef Drosophila Conference, Cairns, Australia |
Membership in committees
Member of the Scientific Board | Faculty of Science, University of South Bohemia, České Budějovice | |
Member of the Board | Grant agency of University of South Bohemia | |
Member of the regional committee | Secondary school vocational activities (SOČ) |
Publications
- Nedbalova P, Kaislerova N, Chodakova L, Moos M, Dolezal T. (2024) SAM transmethylation pathway and adenosine recycling to ATP are essential for systemic regulation and immune response. eLife 13:RP105039. https://doi.org/10.7554/eLife.105039.2
- Kazek M, Chodáková L, Lehr K, Strych L, Nedbalová P, McMullen E, Bajgar A, Opekar S, Šimek P, Moos M, Doležal T. (2024) Glucose and trehalose metabolism through the cyclic pentose phosphate pathway shapes pathogen resistance and host protection in Drosophila. PLOS Biology 22(5): e3002299. https://doi.org/10.1371/journal.pbio.3002299
- McMullen, E., Strych, L., Chodakova, L., Krebs, A., Doležal, T. (2024) JAK/STAT mediated insulin resistance in muscles is essential for effective immune response. Cell Commun Signal 22, 203. https://doi.org/10.1186/s12964-024-01575-0
- Doležal T. (2023) How to eliminate pathogen without killing oneself? Immunometabolism of encapsulation and melanization in Drosophila. Frontiers in Immunology. Dec 6:14:1330312. doi: 10.3389/fimmu.2023.1330312. eCollection 2023.
- Krejčová G., Morgantini C., Zemanová H., Lauschke V.M., Kovářová J., Kubásek J., Nedbalová P., Kamps-Hughes N., Moos M., Aouadi M., Doležal T. & Bajgar A. (2023) Macrophage-derived insulin antagonist ImpL2 induces lipoprotein mobilization upon bacterial infection. The EMBO Journal. Dec 1;42(23):e114086. doi: 10.15252/embj.2023114086. Epub 2023 Oct 9.
- Bajgar A, Krejčová G, Doležal T (2021) Polarization of Macrophages in Insects: Opening Gates for Immuno-Metabolic Research. Front Cell Dev Biol, 15 February 2021 | https://doi.org/10.3389/fcell.2021.629238 (IF=5.2)
- Doležal T, Nedbalová P, Krejčová G, Kazek M, Lehr K, Chodáková L, Strych L, Dolejšková T, Bajgar A (2020) Privileged immune cell upon activation – how it changes its own metabolism and metabolism of the whole organism. figshare POSTER DOI: 10.6084/m9.figshare.12144831.v1
- Krejcova G, Danielova A, Nedbalova P, Kazek M, Strych L, Chawla G, Tennessen JM, Lieskovská J, Jindra M, Dolezal T, Bajgar A (2019) Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense. eLife 14;8. pii: e50414. doi: 10.7554/eLife.50414. (IF=7.5)
- Bajgar A, Salon I, Krejcová G, Dolezal T, Jindra M, Stepanek F (2019) Yeast glucan particles enable intracellular protein delivery in Drosophila without compromising the immune system. Biomater Sci 30. doi: 10.1039/c9bm00539k. (IF=5.2)
- Dolezal T, Krejcova G, Bajgar A, Nedbalova P, Strasser P (2019) Molecular regulations of metabolism during immune response in insects. Insect Biochem Mol Biol 109:31-42. doi: 10.1016/j.ibmb.2019.04.005. (IF=3.9)
- Bajgar A, Dolezal T (2018) Extracellular adenosine modulates host-pathogen interactions through regulation of systemic metabolism during immune response in Drosophila. PLoS Pathog 14(4): e1007022 (IF=6.6)
- Dolezal T (2015) - Adenosine: a selfish-immunity signal? Oncotarget - Immunology and Microbiology Section 6 (32), 32307-32308 (IF=6.3)
- Bajgar A, Kucerova K, Jonatova L, Tomcala A, Schneedorferova I, Okrouhlik J, Dolezal T (2015) Extracellular Adenosine Mediates a Systemic Metabolic Switch during Immune Response. PLoS Biol 13(4): e1002135 (IF=11.8)
- Novakova M and Dolezal T (2011). Expression of Drosophila adenosine deaminase in immune cells during inflammatory response. PLoS ONE 6(3): e17741 (IF=3.2)
- Fenckova M, Hobizalova R, Fric Z, Dolezal T (2011). Functional characterization of ecto-5’-nucleotidases and apyrases in Drosophila melanogaster. Insect Biochem Mol Biol 41(12): 956-967 (IF=3.4)
- Zuberova M, Fenckova M, Simek P, Janeckova L, Dolezal T (2010). Increased extracellular adenosine in adenosine deaminase deficient flies activates a release of energy stores leading to wasting and death. Dis Model Mech 3(11-12): 773-84 (IF=4.9)
- Dolezal T, Kucerova K, Neuhold J, Bryant PJ (2010). Casein kinase I epsilon somatic mutations found in breast cancer cause overgrowth in Drosophila. Int J Dev Biol 54: 1419 – 1424 (IF=2.8)
- Foldynova-Trantirkova S, Sekyrova P, Tmejova K, Brumovska E, Bernatik O, Blankenfeldt W, Krejci P, Kozubik A, Dolezal T, Trantirek L, Bryja V. (2010). Breast cancer specific mutations in CK1epsilon inhibit Wnt/beta-catenin and activate Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration. Breast Cancer Res 12(3): R30 (IF=5.5)
- Dolezal T, Dolezelova E, Zurovec M, Bryant PJ (2005). A Role for Adenosine Deaminase in Drosophila Larval Development. PLoS Biol 3(7): e201 (IF=11.8)
- Dolezelova E, Zurovec M, Dolezal T, Simek P, Bryant, PJ (2005). The emerging role of adenosine deaminases in insects. Insect Biochem Mol Biol 35(5): 381-9 (IF=2.8)
- Dolezal T, Gazi M, Zurovec M, Bryant PJ (2003). Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila. Genetics 165: 653-666 (IF=4.3)
- Zurovec M, Dolezal T, Gazi M, Pavlova E, Bryant PJ (2002). Adenosine deaminase-related growth factors stimulate cell proliferation in Drosophila by depleting extracellular adenosine. PNAS 99(7): 4403-4408 (IF=10.9)
Teaching activities at the University of South Bohemia
Teaching of courses:
KMB / 758 Cellular and Molecular Biology and Genetics - Guarantor and lecturer
KMB / 921 Introduction to Omics & Biotechnology - Guarantor and lecturer
KMB / 924 Molecular Physiology and Metabolism - Guarantor and lecturer
KMB / 603 Advanced methods in Molecular Biology - Guarantor and lecturer
KMB / 926 Seminars in Omics & Biotechnology - seminar
KMB / 023 Fundamentals of Cell Biology - Guarantor and lecturer
KZO / 444 Biology of the Organism - Lecturer
2006-2016 guarantor and lecturer (100%) of “Foundation of modern biology” (undergraduate course)
2007-2010 guarantor and lecturer (100%) of “Molecular biology and Genetics” (undergraduate course in English for international program Biological Chemistry)
2008-2009 guarantor and lecturer (20%) of “Cell regulation and signaling” (graduate course in English)
2010-2012 guarantor and lecturer (100%) of “Basic methods in molecular biology” (undergraduate course)
since 2014 lecturer (50%) of “Developmental biology” (undergraduate course)
since 2017 guarantor and lecturer (100%) of "Introduction to Cell Biology" - mandatory course for Czech undergraduate Biology programme and undergraduate course in English for international program Biological Chemistry
since 2017 guarantor of "Methods in Molecular Biology" (undergraduate course in English for international program Biological Chemistry)
Supervision of students and postdocs
2 Postdocs / 5 Ph.D. students / 6 Master Students / 18 Bachelor Students
Ph.D. theses:
Tereza Dolejšková, ongoing - "Coordination of development and immune competence by systemic hormone signaling mapping the influence of juvenile hormone on hematopoiesis and genetic interactions with other immune pathways"
Pavla Nedbalová, ongoing - "Origin of adenosine in activated immune cells in Drosophila"
Milena Novakova, 2011 - "Regulation of extracellular adenosine level by ADGF-A in Drosophila melanogaster"
Michaela Fenckova, 2012 - "Role of extracellular adenosine in Drosophila"
Monika Zuberova, 2012 - "The role of adenosine signaling pathway in regulation of metabolic reserves"
Master degree theses:
Tereza Dolejšková, 2023 - "The role of Gluconate shunt in the immunity ofDrosophila melanogaster"
Nikola Kaislerová, 2022 - "Úloha Adenylát kinázy 1 v aktivaci a metabolismu imunitních buněk larev Drosophila melanogaster"
Tomas Gregor, 2015 - "Tissue specific mutagenesis with TAL nucleases using Drosophila melanogaster as a model" (in Czech)
Lucie Jonatova, 2013 - "The Role of Dco in Drosophila Haematopoiesis”
Jana Matulova (Neuhold), 2009 - "Study of the Role of Casein Kinase I epsilon in BreastCancer Using Drosophila as a Model"
Supervised Bachelor students:
Jakub Sysel, Jiří Blažek, Hanh My Truong, Klára Kopicová, David Opavsky, Jana Kadlecova, Tomas Krucek, Radka Hobizalova, Eugen Kubala, David Hartmann, Sarka Pospisilova, Tamara Aigner, Bianca Spitzbart, Marek Renner, Kristina Preur, Tomas Gregor, Jiri Stros, Paul Strasser, Klara Kopicova, Thomas Stehrer, Barbara Jetzinger, Lukas Strych, Katharina Lehr
