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Research

1) Papaver somniferum (poppy) as a model crop

a. Poppy characterisation

  1. Poppy photosynthesis / respiration, stomata and cuticle
    Our aim is to perform detailed functional characterisation of poppy photosynthesis (respiration), stomata and cuticle function as at our department we have long tradition in such research. All above-mentioned metabolic pathways and structural components are critical in plant development and responses to abiotic and biotic stresses which is at the end crucial for yield.
    1. Running thesis: Stomata on poppyheads – Marie Petráková
    2. Available thesis topic: Cuticle on leaf of the poppy
  2. Poppy gene editing 
    Our aim is to establish whole protocol for poppy gene editing. It includes poppy transformation and usage of CRISPR based approach
    (also part of the „healthy poppy“ project)
    1. Available thesis topics, in general: Gene editing of Papaver somniferum.

b. „Healthy poppy“ The development of new approaches for poppy protection against pathogens and pests.

  1. Poppy (Papaver somniferum) immunity
    Our aim is to monitor typical plant immune responses in poppy.
    (Orozco Hernández et al. 2025)
  2. Poppy gene editing with the aim to modulate poppy immunity
  3. Establishment of poppy pathosystems
  4. Spraying with dsRNA as an alternative approach for protection of poppy against pathogens and pests
    Our aim is to develop dsRNA with which we will treat poppy pathogens, and the dsRNA will inhibit pathogen growth by RNAi
  5. Early diagnostics of poppy pathogens on the field
    Our aim is to develop system by which the farmers will be able to make molecular diagnostics directly on the field
2) Arabidopsis thaliana as a model plant in study plant immunity

 a. Cuticle and plant immunity

Does triggered plant immunity affects cuticle composition and/or dynamics
(Kalistová and Janda 2023)

b. Salicylic acid signalling pathway in plants

In this project we are mainly focused on the deciphering of the mechanism of the effect of salicylic acid on plant growth and development.
(Drs et al. 2025)

c. Pattern triggered immunity (PTI)

In this project we are closely collaborate with the Kulich laboratory at IMPB BC CAS.

  1. Role of CNG channels (Ca2+) in PTI
    1. Available thesis topic: CNGCs in PTI
  2. The effect of high temperature on PTI
    1. Available thesis topic: The heat stress effect on PTI
3) Service: methods in studying plant immunity

We are offering to perform a screening of plant immunity in any type of collaboration. We have established the methods for Arabidopsis and poppy, but we are open to discuss about collaboration (service) on any plant species)

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